Detection of West Nile Virus in Mosquitoes of Montana through RT-PCR and TaqMan Assays and Correlation to Reservoir Competent Avian Populations

carrollscholars.legacy.contextkey11206102
carrollscholars.legacy.itemurlhttps://scholars.carroll.edu/lifesci_theses/108
carrollscholars.object.degreeBachelor's
carrollscholars.object.departmentLife & Environmental Sciences
carrollscholars.object.disciplinesBiodiversity; Biology; Ecology and Evolutionary Biology; Entomology; Life Sciences
carrollscholars.object.seasonSpring
dc.contributor.advisorSam Alvey
dc.contributor.advisorJennifer Glowienka
dc.contributor.advisorKelly Cline
dc.contributor.authorSemmens, Katie
dc.date.accessioned2020-04-30T10:00:39Z
dc.date.available2020-04-30T10:00:39Z
dc.date.embargo12/31/1899 0:00
dc.date.issued2010-04-01
dc.description.abstractFirst emerging in the United States in 1999, and Montana in 2002, West Nile Virus (WNV) has had a significant impact on human and avian populations. Due to this significance, a greater understanding of the factors affecting the prevalence of WNV is necessary. Mosquitoes were collected from 27 locations across the state of Montana throughout the summer of 2009. RT-PCR and TaqMan assays were used to identify positive pools of mosquitoes, with three locations testing positive. As avian species serve as a reservoir for the virus, it is hypothesized that locations testing positive for WNV should have a greater presence of reservoir competent species. A contingency analysis was run to test for an association between the presence of avian competent species and WNV, resulting in a p-value of 0.425. These results do not establish a statistically significant correlation between presence of reservoir competent species and WNV. However, due to limitations, further analysis is necessary to determine if a significant correlation exists and if other factors are involved.
dc.identifier.urihttps://scholars.carroll.edu/handle/20.500.12647/2851
dc.subjectCulex tarsalis, mosquitoes, West Nile virus, birds
dc.titleDetection of West Nile Virus in Mosquitoes of Montana through RT-PCR and TaqMan Assays and Correlation to Reservoir Competent Avian Populations
dc.typethesis
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