The Effect Of The Linker DNA regions Of Mononucleosomes With Respect To Stability In Thermal Denaturation Analysis

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Authors
Salmassy, David
Advisor
E. Morton Bradbury
James Manion
James Trudnowski
Editor
Date of Issue
1985-04-01
Subject Keywords
Publisher
Citation
Series/Report No.
item.page.identifier
Title
The Effect Of The Linker DNA regions Of Mononucleosomes With Respect To Stability In Thermal Denaturation Analysis
Other Titles
Type
thesis
Description
Abstract
The stability afforded to the mononucleosomal core complex on samples of chicken erythrocyte chromatin was studied. Samples of chromatin digested to mononucleosomal (145 + base pairs) and core particle lengths (145 base pairs) were used in a nonreconstituted and a reconstituted form using High Mobility Group protein 17 in a series of thermal denaturation melting studies. The results give evidence that the linker DMA regions extending into and out of the entry and exit sites of the mononucleosome may indeed enhance the stability of the complex against the melting. This can not be stated as an isolated fact because of the presence of histone protein Hi in the large sample fractions which may independently have its own effect on the stability of the complex
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences