Date of Award

Winter 2019

Document Type

Thesis

Department

Health Sciences

First Advisor

Alyssa Hahn

Second Advisor

Brandon Sheafor

Third Advisor

Kelly Parsley

Abstract

Objectives: The objective of this study was to investigate the acute metabolic response to injury using global, or untargeted, metabolic profiling. The purpose was to better understand the acute response to injury following a second traumatic joint injury. Methods: This study is a case report of a patient who had sustained two separate ACL tears in the same knee years apart. We extracted metabolites from healthy synovial fluid and human joint fluid following the patient’s second ACL tear and analyzed the metabolite extracts using liquid chromatography-mass spectrometry (LC-MS)-based global metabolic profiling. Results: We identified 1241 unique metabolite features in injured SF compared to healthy SF (Student’s t-test: FDR-adjusted p-value

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