• Login
    View Item 
    •   Carroll Scholars Home
    • Carroll College Student Undergraduate Research Festival
    • Carroll College Student Undergraduate Research Festival 2018-2019
    • View Item
    •   Carroll Scholars Home
    • Carroll College Student Undergraduate Research Festival
    • Carroll College Student Undergraduate Research Festival 2018-2019
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Effect of Turmeric on Oxidatively Stressed Tetrahymena thermophila Cells

    Thumbnail
    View/Open
    Meeks_Logan_SURF2019.pdf (328.4Kb)
    Author
    Meeks, Logan; McEntee, Luke
    Date of Issue
    2019-04-25
    Metadata
    Show full item record
    URI
    https://scholars.carroll.edu/handle/20.500.12647/7253
    Title
    Effect of Turmeric on Oxidatively Stressed Tetrahymena thermophila Cells
    Abstract
    Reactive oxygen species (ROSs) are chemically reactive forms of oxygen that, within a cellular environment, form free radicals that interfere with cellular processes and damage DNA. ROSs are routinely produced during oxidative metabolic processes that occur within the mitochondria and their presence in the cell must be tightly regulated. The OXR1 gene encodes a protein that is essential to the degradation of ROSs primarily in the nucleus and in the mitochondria. In this study, we wanted to determine whether turmeric had a protective effect on ROS-induced oxidative stress through regulation of the OXR1 gene in the model organism Tetrahymena thermophila. Turmeric is a spice produced from a species of ginger root that has been used for centuries as an antioxidant and anti-inflammatory agent. We hypothesized that turmeric would have a negligible effect on OXR1 gene expression and cell growth in Tetrahymena that were experiencing high levels of ROS-induced oxidative stress. To test our hypothesis, experimental groups of Tetrahymena cells were exposed to the oxidative stress inducer oligomycin for one week in the presence of turmeric extract while control groups of cells were treated solely with oligomycin. During the week-long exposure, Tetrahymena cells were counted every 24 hours to determine growth grate. At the end of the exposure, RNA was extracted from the control and experimental groups and Reverse Transcription quantitative Polymerase Chain Reaction (RT-qPCR) was performed to measure the expression of OXR1. We predicted that turmeric would have no effect on OXR1 expression and cell growth in Tetrahymena thermophila.
    Collections
    • Carroll College Student Undergraduate Research Festival 2018-2019

    Browse

    All of Carroll ScholarsCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    DSpace software copyright © 2002-2023  DuraSpace
    DSpace Express is a service operated by 
    Atmire NV