• Login
    View Item 
    •   Carroll Scholars Home
    • Carroll College Student Undergraduate Research Festival
    • Carroll College Student Undergraduate Research Festival 2018-2019
    • View Item
    •   Carroll Scholars Home
    • Carroll College Student Undergraduate Research Festival
    • Carroll College Student Undergraduate Research Festival 2018-2019
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    The Effects of Roundup on Tetrahymena thermophila Growth and Cytochrome p450 Gene Expression

    Thumbnail
    View/Open
    Viles_Ethan_SURF2019.pdf (509.8Kb)
    Author
    Viles, Ethan; Walker, Henry
    Date of Issue
    2019-04-25
    Metadata
    Show full item record
    URI
    https://scholars.carroll.edu/handle/20.500.12647/7247
    Title
    The Effects of Roundup on Tetrahymena thermophila Growth and Cytochrome p450 Gene Expression
    Abstract
    Roundup is a very common herbicide in the United States, but has been linked to many detrimental health effects and was classified as ‘probably carcinogenic’ by the World Health Organization in 1994. Not much research has been done to elucidate the possible effects on non-cancer gene pathways.The goal of this project was to research the influence that exposure to Roundup has on the growth of Tetrahymena thermophila and the expression of the cytochrome p450 gene. It was hypothesized that Roundup exposure would change the expression of cytochrome p450 gene and would decrease the growth rate of Tetrahymena thermophila. Background research indicated Roundup affected cytochrome p450 gene expression in human placental JPG3 cells that codes for the NADPH-dependent reductase. NADPH-dependent reductase is critical for the synthesis of estrogen and other sex hormones. For the experiment, Tetrahymena was treated with a predetermined dose of Roundup, and growth was measured every 12 hours for 72 hours. After the treatment, RNA was extracted from both a control group and the experimental group treated with Roundup. Real-time quantitative PCR was used in order to determine the level of expression of cytochrome p450.
    Collections
    • Carroll College Student Undergraduate Research Festival 2018-2019

    Browse

    All of Carroll ScholarsCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    DSpace software copyright © 2002-2023  DuraSpace
    DSpace Express is a service operated by 
    Atmire NV