• Login
    View Item 
    •   Carroll Scholars Home
    • Life and Environmental Sciences
    • Life and Environmental Sciences Course-based Research Projects
    • View Item
    •   Carroll Scholars Home
    • Life and Environmental Sciences
    • Life and Environmental Sciences Course-based Research Projects
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    The Effect of Basic Conditions on CDK1 Expression in Tetrahymena thermophila

    Thumbnail
    View/Open
    Kaitlin_Jakob_Paper.pdf (70.26Kb)
    Author
    Stromberg, Kaitlin; Plagenz, Jakob; Otto-Hitt, Stefanie
    Advisor
    Stefanie Otto-Hitt
    Date of Issue
    2018-07-01
    Metadata
    Show full item record
    URI
    https://scholars.carroll.edu/handle/20.500.12647/3366
    Title
    The Effect of Basic Conditions on CDK1 Expression in Tetrahymena thermophila
    Type
    paper
    Abstract
    The goal of this project was to discover whether expression of the CDK1 gene in Tetrahymena thermophila, as well as their growth, are aected by changing the pH of the growth media. We hypothesized that expression of the CDK1 gene would decrease significantly when grown in basic media. The CDK1 gene encodes the protein Cyclin-Dependent Kinase, which functions to advance cells through various stages of the cell cycle. To test our hypothesis, Tetrahymena cultures were randomly assigned to either the control group, which was grown in media with a neutral pH, or a treatment group, which was grown in media with a basic pH of 8.5. Previous experiments showed that Tetrahymena were able to survive in pH levels up to 9.0, although their optimal survival was at a neutral pH. The control and experimental cultures were maintained for one week before being processed. Following the weeklong incubation, RNA extraction, reverse transcription, and PCR were conducted on the control and experimental groups to measure expression of CDK1. In order to look at growth rate, we used a hemocytometer to perform cell counts throughout the weeklong incubation. Because CDKs are a major component of the cell cycle, we predicted that expression of the CDK1 gene would decrease, and that growth rate would also decrease, in Tetrahymena cells cultured in a basic environment.
    Semester
    Summer
    Department
    Life and Environmental Sciences
    Collections
    • Life and Environmental Sciences Course-based Research Projects

    Browse

    All of Carroll ScholarsCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    DSpace software copyright © 2002-2022  DuraSpace
    DSpace Express is a service operated by 
    Atmire NV