Microsatellite Analysis of Culex tarsalis Populations in Montana

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Authors
Stromberg, Kaitlin
Advisor
Jennifer Glowienka
Theresa McHugh
William Parsons
Editor
Date of Issue
2019-04-01
Subject Keywords
West Nile Virus, WNV, Culex tarsalis, mosquitos, vector
Publisher
Citation
Series/Report No.
item.page.identifier
Title
Microsatellite Analysis of Culex tarsalis Populations in Montana
Other Titles
Type
thesis
Description
Abstract
West Nile Virus (WNV) has been present in Montana since 2002. The primary vector of WNV in Montana is Culex tarsalis. At Carroll College, the purpose of the WNV project is to survey for the presence of the virus across the state and create a risk model in real time. The goal of this particular study was to find a microsatellite protocol that consistently works in order to create a reliable landscape genetics model for C. tarsalis populations. Three reagent sets were used in order to test which reagent conditions best amplified the three loci, followed by gel electrophoresis to visualize the amplification. The results indicated degradation of the DNA, suggesting new DNA is needed to test which conditions produced the best amplification.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences