Immunological and Immunohistochemical Methods Used to Study Chromatin Neoantigens of Novikoff Hepatoma

Abstract

The micro-complement fixation test was used to assay the potency and specificity of rabbit antisera to chromosomal antigens from Novikoff hepatoma and normal rat liver. The complement fixation titration patterns suggest the presence of only a few species of molecules bearing neochromatin antigens. A immunofluorescent technique was adapted to Novikoff hepatoma cells and resulted in the positive staining of Novikoff chromatin and negligible staining in normal rat liver cells.