The Effects Of Indomethacin On The Biological Activities Of Bacterial LPS Treated Mice

Abstract

C3HeB/FeJ and RML Swiss-Webster mice were given various doses of indomethacin. Concentrations of 500 ug/mouse and greater proved to be toxic when injected 3 times a week. These strains of mice were pretreated with indomethacin and given 2 MLD50 doses of Salmonella Minnesota 1114 WT bacterial LPS. The indomethacin had no effect on the lethal effects of bacterial LPS. RML mice, pretreated with indomethacin, were given a primary and secondary injection of E.Coli 0113 bacterial LPS. The indomethacin had no effect on the ability of RML mice to elicit normal humoral antibody responses, although the indomethacin treated mice showed a higher secondary peak titer than the control mice; this was clinically significant.