• Login
    View Item 
    •   Carroll Scholars Home
    • Life and Environmental Sciences
    • Life and Environmental Sciences Undergraduate Theses
    • View Item
    •   Carroll Scholars Home
    • Life and Environmental Sciences
    • Life and Environmental Sciences Undergraduate Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Purification And Characterization Of Ribouridylyl Terminal Transferase And Ribocytidylyl Terminal Transferase Activities From RNA Tumor Virus-Infected Avian Myeloblasts

    Thumbnail
    View/Open
    1982_DoughertyS_THS_0001085.pdf (3.602Mb)
    Author
    Dougherty, Sarsfield; Harrington, Daniel
    Advisor
    James Manion; John Christenson; Kenneth Watson
    Date of Issue
    1982-04-01
    Metadata
    Show full item record
    URI
    https://scholars.carroll.edu/handle/20.500.12647/3194
    Title
    Purification And Characterization Of Ribouridylyl Terminal Transferase And Ribocytidylyl Terminal Transferase Activities From RNA Tumor Virus-Infected Avian Myeloblasts
    Type
    thesis
    Abstract
    Two enzymes, one which catalyzes the addition of one or more cytidine 5'-monophosphate residue from cytidine 5’-triphosphate to the 3'-terminus of a ribonucleic acid primer, referred to as ribocytidylyl terminal transferase, and one which catalyzes the addition of one or more uridine 5’-monophosphate residue from uridine 5'-triphosphate to the 3'-terminus of a ribonucleic acid primer, referred to as ribouridylyl terminal transferase, have been purified from RNA tumor virus-infected avian myeloblasts. The purification procedure involved cation and anion exchange chromatography. Both enzymes were stimulated by the divalent cations Mg++ and Mn++ with optimal concentrations of 0.0001 M Mn++ and between 0.001 M and 0.004 M Mg++. The molecular weight of ribocytidylyl terminal transferase was approximately 60,000 and the molecular weight of ribouridylyl terminal transferase was approximately 56,000.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
    Collections
    • Life and Environmental Sciences Undergraduate Theses

    Browse

    All of Carroll ScholarsCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    DSpace software copyright © 2002-2023  DuraSpace
    DSpace Express is a service operated by 
    Atmire NV