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    The Effects Of Various Enzymatic And Chemical Treatments On The Infectivity Of Aleutian Disease Virus Of Mink

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    1982_MyhreB_THS_0001075.pdf (2.952Mb)
    Author
    Myhre, Beth
    Advisor
    John Christenson; Rev. Joseph Harrington; Henry Burgess
    Date of Issue
    1982-04-01
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/3190
    Title
    The Effects Of Various Enzymatic And Chemical Treatments On The Infectivity Of Aleutian Disease Virus Of Mink
    Type
    thesis
    Abstract
    Aleutian disease virus (ADV), a parvovirus, was treated with the enzymes trypsin, alpha-chymotrypsin (CT), pronase, deoxyribonuclease (DNase), and ribonuclease (RNase) by incubation at 37C for 60 min. Effects of enzyme treatment on the infectivity of ADV were determined by direct immunofluorescent assay, which showed the only significant drop in titer, about 2 logQ, from treatment with pronase, trypsin, and CT. A change of over 1 log-,g was found to be significant using this assay. A time course of digestion by trypsin and CT was followed through liquid scintillation counting (LSC) of S methionine-labeled Crandall feline kidney cells (CRFKs), both ADV-infected and ADV-free. A time course of ADV digestion by trypsin and x.CT was also followed by direct immunofluorescence assay. Activity of the enzymes on the ADV is most pronounced in the first 10 min of incubation. The results of this experiment concur with those obtained by Burger (8) and Eklund (13) using an in vivo assay method.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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