Analysis Of Mouse Sperm Protamine And The Development Of A Method For The Isolation Of Its mRNA

Abstract

Tritium-labeled protamine was extracted from mouse caudal epidymides. Partial purification of the two protamine components was achieved using a series of chromatographic columns. It was determined that gel electrophoresis was not an adequate method for separating the two components for quantification. Mouse testes were used as a source of protamine mRNA. Procedures to isolate the protamine mRNA included utilization of a DEAE anion exchange column and an Oligo-Dt column. Results indicated that mRNA was not being recovered from the Oligo-Dt column.