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    Analysis Of Mouse Sperm Protamine And The Development Of A Method For The Isolation Of Its mRNA

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    1982_StatesP_THS_0001068.pdf (2.621Mb)
    Author
    States, Patti
    Advisor
    John Christenson; James Manion; Barry Ferst
    Date of Issue
    1982-04-01
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/3186
    Title
    Analysis Of Mouse Sperm Protamine And The Development Of A Method For The Isolation Of Its mRNA
    Type
    thesis
    Abstract
    Tritium-labeled protamine was extracted from mouse caudal epidymides. Partial purification of the two protamine components was achieved using a series of chromatographic columns. It was determined that gel electrophoresis was not an adequate method for separating the two components for quantification. Mouse testes were used as a source of protamine mRNA. Procedures to isolate the protamine mRNA included utilization of a DEAE anion exchange column and an Oligo-Dt column. Results indicated that mRNA was not being recovered from the Oligo-Dt column.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
    Collections
    • Life and Environmental Sciences Undergraduate Theses

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