Preparation Of Chronic Lymphocytic Leukemic Chromosomes For Viewing With The Scanning Electron Microscope

Abstract

Methods of preparing chromosomes from human lymphocytes for viewing with the scanning electron microscope (SEM) were investigated. Several fixatives were used. Chromosomes were isolated on nuclear pore filters, critical point dried, and coated with gold for SEM. Chromosome spreads were not very clear and chromosome overlap prevented establishment of clean, complete karyotypes.