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    Comparison Of Three Strains Of Contagious Equine Metritis Organism By Polyacrylamide Gel Electrophoresis And Description Of Three Colonial Opacity Variants

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    1984_OlszewskiA_THS_0001009.pdf (5.293Mb)
    Author
    Olszewski, Albert
    Advisor
    James Manion; Fr. Joseph Harrington; Barry Ferst
    Date of Issue
    1984-04-01
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/3152
    Title
    Comparison Of Three Strains Of Contagious Equine Metritis Organism By Polyacrylamide Gel Electrophoresis And Description Of Three Colonial Opacity Variants
    Type
    thesis
    Abstract
    The colonial morphology and molecular composition of 26 phenotypes of Contagious Equine Metritis Organisms (CEMO) were studied by stereomicroscopic examination and polyacrylamide gel electrophoresis. Twenty-three phenotypes had colonies that were round and smooth. Three phenotypes had colonies that were round and smooth with occasional fimbria projecting from the edge of the colonies. The consistency of all the colonies varied from friable and butyrous to viscid. Based on external observation in reflected and transmitted light, the 26 phenotypes were separated into 3 different types of colonial variants based on opacity. The major stained bands in the page profiles of all 26 phenotypes were observed and recorded. The stained bands were observed in polyacrylamide 125 gels that were prepared with CBB stain, silver stain, Isurface labeling, and Immuno-electroblotting procedures. Several differences between the polyacrylamide gel profiles of these strains were observed.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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