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    A Comparative Analysis Of Aleutian Disease Virus Strains: Protein Expression And DNA Similarity In ADV Plasmids

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    1984_TierneyG_THS_0001002.pdf (3.448Mb)
    Author
    Tierney, Gregory
    Advisor
    John Christenson; James Manion; Henry Burgess
    Date of Issue
    1984-04-01
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/3148
    Title
    A Comparative Analysis Of Aleutian Disease Virus Strains: Protein Expression And DNA Similarity In ADV Plasmids
    Type
    thesis
    Abstract
    Comparative studies of protein expression and DNA similarity were carried out on two recombinant plasmids of Aleutian Disease Virus. ADV-G genome molecularly cloned into plasmid pUC8 is designated pBMl and a Danish isolate of ADV(DK-ADV) also cloned into pUC8 is designated pBM2. The recombinant plasmid pBMl induced expression of three previously recognized and one unrecognized polypeptides in E. coli strain JM103 that were specifically detected by sera of ADV infected mink. The proteins with approximate molecular weights of 2?K, 36K, 49K, and 58K daltons were detected by immune blotting of JMlO3(pBMl). The same set of proteins was detected in JMlO3(pEM2). No specific proteins were seen in JM103 or JMlO3(pUC8) reacted with Mink AD Sera(MAD) or in JMlO3(pBMl) and JMlO3(pEM2) reacted with Normal Mink Sera(NMS) Comparative study of the genomes of the isolates by restriction enzyme analysis, however, revealed a dissimilarity. Both inserts consisted of an EcoRI-Hindlll fragment replacing a smaller segment of the pUC8 plasmid with complementary ends. Similarity of protein expression suggests similar genomes, but digestion with various restriction endonucleases followed by resolution on agarose gel electrophoresis showed dissimilarities between the DNA with the pBM2 insert containing one more restriction site than the pBMl. These studies seemed to indicate the two isolates were genetically different forms of the Aleutian Disease Virus yet code for the same antigenic determinants.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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