A Study Of S-1 Subunit Specific Monoclonal Antibodies On Digested Whole Pertussigen: The Major Endotoxin Of Bordetella pertussis

Abstract

Common electophoretic and electroblot methods were used to differentiate between possibly different epitopes on digested whole pertussigen. In this study, S-l subunit specific monoclonal antibodies were used. The results of these tests indicate that these monoclonal antibodies react with the same antigenic region and do not distinguish between any other epitopes that may be present on the S-l subunit. Further development and testing of monoclonal antibodies against the various subunits of pertussigen will be required to elucidate the nature and presence of other epitopes of pertussigen.