The Use Of Enzyme-Linked Immunosorbent Assays To Detect Antibodies Of The Colorado Tick Fever Virus

Abstract

An enzyme-linked immunosorbent assay was attempted to determine antibody titers of serum from potential Colorado tick fever victims. An exemplary assay of influenza A monoclonal antibodies worked very well. After turning to the elucidation of optimal reagent purifications and titrations, a high degree of non-specificity was found. The numerous non-specific reactions which were observed necessitate the need for further development of the technique by the State of Montana Health and Environmental Sciences virology department.