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    Isolation Of Horse Large Granular Lymphocytes By Density Centrifugation Techniques, And Responsiveness To Human Interleukin-2

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    1985_MacNeillS_THS_000985.pdf (4.968Mb)
    Author
    MacNeill, Susan
    Advisor
    James Manion; Jean Smith; Allen Pope
    Date of Issue
    1985-04-01
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/3135
    Title
    Isolation Of Horse Large Granular Lymphocytes By Density Centrifugation Techniques, And Responsiveness To Human Interleukin-2
    Type
    thesis
    Abstract
    The methods of isolating human and rat large granular lymphocytes were reviewed, and revised to accommodate horse lymphocytes. Purified populations of large granular lymphocytes were then stimulated with Interleukin-2 and Concanavalin-A, to observe effects on proliferation and cytotoxic activity. The results of this study suggest that large granular lymphocytes can be purified from the peripheral blood of horses, using a percoll density gradient. Also, Interleukin-2 is shown to have an effect on proliferation of enriched populations of large granular lymphocytes, especially when added in adjunct to Concanavalin-A.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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