Illucidation Of Serum Gamma Globulins By Two-Dimensional Immunoelectrophoresis With Isogel

Abstract

This research project was designed to examine new possibilities for adapting two-dimensional immunoelectrophoresis to clinical situations, much as has been done with standard one-dimensional electrophoresis over the past several years. The hypothesis was that by using a gel medium with relatively no electroendosmosis, the immunoglobulins would become visible for evaluation with two-dimensional techniques. Results show that, although more work is necessary to refine the process, the immunoglobins do, indeed, become visible in two-dimensional assay procedures using this gel. This method shows a great deal of promise for future clinical acceptance of two-dimensional electrophoretic techniques.