Construction Of Escherichia Coli Mapping Strains And Mapping Of The Lysine-Decarboxylase Gene CAD A

Abstract

The Escherichia coli lysine-decarboxylase gene, cadA, was mapped using the mutant form and two and three factor Crosses. The three point analysis showed the gene order of the markers to be me1A - cadA - groE. Two factor crosses yielded cotransduction frequencies which were compared to results from other mapping experiments. After considering the length of insertion element TnlO, the agreement with pub dished' data was very good. Map distances were calculated from the cotransduction frequencies. The significance of this experiment lies in the fact that it mapped the cadA gene more closely and conclusively than had been done previously. Markers on both sides of the gene were used and map distances were calculated.