A Study Of The Determinants On GP70, A Viral Envelope Glycoprotein, To Which Neutralizing Monoclonal Antibodies Bind

Abstract

Lake Casitas Ecotropic virus causes a hind limb paralysis when injected into newborn mice. The long term goal is to select mutants of the virus using monoclonal antibodies and mutagens. Two of the monoclonal antibodies produced against this virus are neutralizing and also bind to the protein part of gp70, a viral envelope glycoprotein. Because of this, they can be used to select mutant viruses. There is no significant binding inhibition between monoclonal 704 and either of the two neutralizing monoclonals. Therefore, monoclonal 704 can be used in a focal-immuno-assay with the neutralizing monoclonals to locate mutant viruses.