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    Isoelectric Focusing And Immunofixation Of Serum Proteins In An Agarose Gel Support Medium

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    1986_MinnerlyL_THS_000956.pdf (2.872Mb)
    Author
    Minnerly, Lorinda
    Advisor
    James Manion; John Christenson; Richard Lambert
    Date of Issue
    1986-04-01
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/3116
    Title
    Isoelectric Focusing And Immunofixation Of Serum Proteins In An Agarose Gel Support Medium
    Type
    thesis
    Abstract
    A viable procedure was sought for agarose gel electrophoresis and isoelectric focusing of human serum proteins. Normal control serum was obtained and compared with a pi marker through isoelectric focusing. Immunofixation of the separated proteins was accomplished by overlaving of specific antisera, which caused an antigen-antibody precipitant. The protocol was standardized using a series of trial dilutions and can now be used in detection of serum protein deficiencies and disorders, and immunoglobulin deficiency diagnosis. The clinical applications of this procedure will have far reaching effects.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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