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    Isolation & Partial Characterization Of The Capsule Produced By Neisseria gonorrhoeae

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    1986_SullivanP_THS_000947.pdf (4.979Mb)
    Author
    Sullivan, Patricia
    Advisor
    James Manion; John Christenson; Barry Ferst
    Date of Issue
    1986-04-01
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/3110
    Title
    Isolation & Partial Characterization Of The Capsule Produced By Neisseria gonorrhoeae
    Type
    thesis
    Abstract
    The capsule of Neisseria gonorrhoeae strains JS1 and JS3 was extracted according to the procedure of Wolfgang Casper (1937). The extracted products were biologically and biochemically characterized. The extracts were comprised primarily of carbohydrate and other constituents detected are ribonucleic acids, protein, 1ipopolysaccharide, phosphorus, and 2-keto-3-deoxyoctonate. The presence of these impurities has greatly interfered with the identification of the specific polysaccharide components. Examination of Neisseria gonorrhoeae by scanning electron microscopy indicated that extracellular material was stabilized by low temperatures. In order to maximize the amount of extracted polysaccha ride, the bacterial suspensions were refrigerated whenever possible during the purification procedure.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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