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    Benzo (a) Pyrene Monitoring In Small Terrestrial Mammals

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    1987_FeldkampT_THS_000933.pdf (4.387Mb)
    Author
    Feldkamp, Teresa
    Advisor
    John Christenson; Jean Smith; Jack Semmens
    Date of Issue
    1987-04-01
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/3103
    Title
    Benzo (a) Pyrene Monitoring In Small Terrestrial Mammals
    Type
    thesis
    Abstract
    Small terrestrial vertebrates were trapped at three sites near Oak Ridge, Tennessee: East Fork Poplar Creek (EFPC) floodplain, White Oak Lake, and the ORNL steam plant. The sediment of the floodplain at EFPC was determined to be contaminated by polycyclic aromatic hydrocarbons (PAH's). Blood of the animals trapped was analyzed by HPLC/fluorescent spectrophotometry for benzo (a) pyrene (BaP), a carcinogenic PAH, to detect the movement of the BaP from the contaminated soil of the floodplain to the animals inhabiting the area. Chromatographs of blood from four white-footed mice (Peromvscus leucopus). two shorttail shrews (Blarina brevicauda). three cotton rats (Siamodon hispidus). and an Eastern box turtle (Terrepene Carolina Carolina) showed peaks of a substance whose retention time from the HPLC column was slightly longer than the retention time of the standard BaP tetrol peak. These peaks may represent BaP bound to impurities in the sample, causing the retention time to be lengthened slightly. However, further analysis must be done to determine if the peaks present are actually BaP. Modes of uptake of the BaP through the food chain or by soil contact are discussed
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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