• Login
    View Item 
    •   Carroll Scholars Home
    • Life and Environmental Sciences
    • Life and Environmental Sciences Undergraduate Theses
    • View Item
    •   Carroll Scholars Home
    • Life and Environmental Sciences
    • Life and Environmental Sciences Undergraduate Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    A Determination Of The Placental Permeability Of Morphine-3-Glucuronide In Guinea Pigs Using High Pressure Liquid Chromatography With Ultra-Violet Detection

    Thumbnail
    View/Open
    1987_GrossoS_THS_000932.pdf (3.745Mb)
    Author
    Grosso, Steven
    Advisor
    James Manion; Guido Bugni; Richard Lambert
    Date of Issue
    1987-04-01
    Metadata
    Show full item record
    URI
    https://scholars.carroll.edu/handle/20.500.12647/3102
    Title
    A Determination Of The Placental Permeability Of Morphine-3-Glucuronide In Guinea Pigs Using High Pressure Liquid Chromatography With Ultra-Violet Detection
    Type
    thesis
    Abstract
    Morphine (M) is conjugated to glucuronic acid in the fetus. This metabolite, morphine-3-glucuronide (M3G), is less lipid soluble and larger than M. Because of the potential for fetal accumulation (1) and toxicity (2) the permeability for M3G was examined in the guinea pig which, like the human, has a hemomonochorial placenta. Seven dams in the last half of gestation and 30 fetuses were studied. A carotid artery catheter was placed in the anesthetized dam. Two hours later, M3G was injected into the catheter of the awake animal and maternal samples were taken at intervals for 30 minutes. The dam was re-anesthetized and fetal samples taken from the umbilical vein. Plasma M3G was determined by High Pressure Liquid Chromatography (HPLC). Using Fick's law of diffusion, the permeability surface area product (P.S.) per gram placental weight was calculated to be 3.1 X 10'5± 0.3 ml sec '1 g'1 (mean ± S.E.). PS increased with increasing fetal weight. Although the placenta becomes more permeable to M3G as gestation progresses, the permeability of this hydrophilic metabolite is restricted as term approaches and therefore M3G would accumulate in the fetus during chronic morphine exposure, due to conjugation of fetal M to M3G.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
    Collections
    • Life and Environmental Sciences Undergraduate Theses

    Browse

    All of Carroll ScholarsCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    DSpace software copyright © 2002-2023  DuraSpace
    DSpace Express is a service operated by 
    Atmire NV