The Effects Of Attenuated Bovine Viral Diarrhea Virus On Cultured Bovine Fetal Cells

Abstract

Lung, kidney, intestine, aorta, turbinate epithelium, endothelium, cornea, and spleen were extracted from bovine fetuses. These cells were grown into monolayers and inoculated with serial dilutions of BVD virus. After a 96-hr period, the effects of BVD virus upon these cell cultures were studied. Our results demonstrated a difference in the susceptibility of these cell cultures towards BVD virus. The intestinal cells were the most susceptible to BVD viral infection and the least susceptible were cornea cells. There was a 4.5-fold difference in the amount of viral particles needed to cause infection.