The Study Of The Effects Of 60-HZ Electric Fields On The Cloning Efficiencies Of Chinese Hamster Ovary Cells
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Authors
Popp, Julie
Advisor
James Manion
William Wood
Rev. Eugene Peoples
William Wood
Rev. Eugene Peoples
Editor
Date of Issue
1987-04-01
Subject Keywords
Publisher
Citation
Series/Report No.
item.page.identifier
Title
The Study Of The Effects Of 60-HZ Electric Fields On The Cloning Efficiencies Of Chinese Hamster Ovary Cells
Other Titles
Type
thesis
Description
Abstract
Chinese hamster ovary (CHO) cells were exposed to 60-Hz electric fields at 18 V/m and 5 V/m, and the cloning efficiencies compared. Statistical studies failed to show a significant dose response for the field values tested. However, with regard to the 5 V/m field, a trend of 85% viability compared to sham-exposed cells was discovered. In general, cloning efficiencies for sham-exposed controls were low. Various techniques were employed to increase the efficiencies of the controls. Suggestions for improving controls and the overall procedure are discussed.
Sponsors
Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences