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    The Study Of The Effects Of 60-HZ Electric Fields On The Cloning Efficiencies Of Chinese Hamster Ovary Cells

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    1987_PoppJ_THS_000918.pdf (2.570Mb)
    Author
    Popp, Julie
    Advisor
    James Manion; William Wood; Rev. Eugene Peoples
    Date of Issue
    1987-04-01
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/3095
    Title
    The Study Of The Effects Of 60-HZ Electric Fields On The Cloning Efficiencies Of Chinese Hamster Ovary Cells
    Type
    thesis
    Abstract
    Chinese hamster ovary (CHO) cells were exposed to 60-Hz electric fields at 18 V/m and 5 V/m, and the cloning efficiencies compared. Statistical studies failed to show a significant dose response for the field values tested. However, with regard to the 5 V/m field, a trend of 85% viability compared to sham-exposed cells was discovered. In general, cloning efficiencies for sham-exposed controls were low. Various techniques were employed to increase the efficiencies of the controls. Suggestions for improving controls and the overall procedure are discussed.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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