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    Smooth Muscle Cell Mitogen Production By Endothelial Cells In The Lumens Of Healing Synthetic Vascular Prostheses: A Possible Role In Healing

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    1987_ReevesG_THS_000917.pdf (3.938Mb)
    Author
    Reeves, Gary
    Advisor
    Rev. Joseph Harrington; Jean Smith; Richard Lambert
    Date of Issue
    1987-04-01
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/3094
    Title
    Smooth Muscle Cell Mitogen Production By Endothelial Cells In The Lumens Of Healing Synthetic Vascular Prostheses: A Possible Role In Healing
    Type
    thesis
    Abstract
    Media was conditioned by culturing endothelial or medial smooth muscle cells in DME-F12 or by time-course perfusions of segments of aorta, synthetic polytetrafluoroethylene (PTFE) arterial grafts with an intact endothelium, and deendothelialized FIFE grafts. These conditioned media samples were used as culture media for quiescent medial smooth muscle cells. Mitogenesis assays were conducted using radioactive thymidine labeling to test each medium for its ability to stimulate the quiescent smooth muscle cells to enter the S phase from the G^Gq phase. Ratios of the thymidine incorporation of cells cultured in conditioned media to those of the negative control, which £ lacked mitogens, indicated that healing synthetic grafts with an intact endothelium produced greater levels of mitogens than did undamaged aortas. Attempts, using antibodies to Platelet Derived Growth Factor, a known smooth muscle cell mitogen, to test whether the mitogens produced in the grafts was PDGF indicated that further purification of the antibodies was needed. The possible relationship between mitogen production in arteries and healing synthetic grafts is discussed.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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