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    Genetic Diversity of Dermacentor andersoni in Western Montana via Inter-simple Sequence Repeats

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    HensleyN_2018_Final.pdf (458.1Kb)
    Author
    Hensley, Nicholas
    Advisor
    Jennifer Glowienka; Grant Hokit; Edward Glowienka
    Date of Issue
    2018-04-01
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/3084
    Title
    Genetic Diversity of Dermacentor andersoni in Western Montana via Inter-simple Sequence Repeats
    Type
    thesis
    Abstract
    Demacentor andersoni (Ixodidae) is one of the most important disease vectors in Montana. Ticks transmit more animal disease agents than any other blood-sucking arthropods. Ticks are the second most important vector in public health and the most important in veterinary medicine. Dermacentor andersoni is the vector of Rocky Mountain spotted fever, Colorado tick fever, tularemia, bovine anaplasmosis, and Powassan encephalitis. Population genetic studies of disease vectors can allow insight into the vectors contribution to the spread of disease and possible control strategies. This study used inter-simple sequence repeats (ISSRs) to assess genetic variation within and among populations. An analysis of molecular variance (AMOVA) and population pairwise fixation index (FST) were used to determine statistical differences in ISSR banding patterns. A regression analysis was performed to determine if there was a significant relationship between Euclidean distance and FST values. Significant variation was found between individuals and populations, though no significant variation was found between groups of populations. While no connection was found between geography and genetic variation, this analysis was limited by small population sizes for some sites. This analysis found that ISSRs could potentially be useful in determining genetic variation in D. andersoni populations.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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