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    Morphological And Cytochemical Characterization Of Rat Adrenocortical Cells in vitro

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    1988_McGeehanD_THS_000894.pdf (2.390Mb)
    Author
    Geehan, Daniel
    Advisor
    John Addis; Jean Smith; Ruth Carrington
    Date of Issue
    1988-04-01
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/3072
    Title
    Morphological And Cytochemical Characterization Of Rat Adrenocortical Cells in vitro
    Type
    thesis
    Abstract
    Primary monolayer cultures of adrenocortical cells were established from adult female rats of the Wistar strain by single treatment with collagenase type II. This lot of collagenase had been evaluated for collagenase, clostripain, caseinase, and tryptic enzyme activities. The adrenocortical cells were maintained in Dulbecco's Modified Eagle's Medium, Fetal Calf Serum, and gentamycin. Morphological observation of cultures revealed the presence of epithelial cortical cells and absence of medullary cells (epithelial or fibroblastic). The cells contained cytoplasmic lipid granules. In an attempt to localize sites of 3 Beta - hydroxysteroid dehydrogenase activity, cytochemical experiments were performed.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
    Collections
    • Life and Environmental Sciences Undergraduate Theses

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