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dc.contributor.advisorJohn Christenson
dc.contributor.advisorRev. Joseph Harrington
dc.contributor.advisorRev. J. Eugene Peoples
dc.contributor.authorMorin, Lisa
dc.date.accessioned2020-04-30T10:02:09Z
dc.date.available2020-04-30T10:02:09Z
dc.date.issued1988-04-01
dc.identifier.urihttps://scholars.carroll.edu/handle/20.500.12647/3070
dc.description.abstractA pilot experiment was performed in which peripheral blood lymphocytes were isolated from whole blood using the Ficoll-Hypaque technique. Percoll discontinuous-gradient centrifugation of peripheral blood mononuclear cells resulted in a 2-fold increase in natural killer cell activity coupled with a 3 to 4-fold increase in large granular lymphocytes. Morphology, function (NK activity), and phenotype of Percoll fractions 2-4, presumably large granular lymphocytes, were compared with that of lower density fractions 6-7 over a 28-day period. Augmentation agents phytohemagglutinin and recombinant interleukin-2 were added to the culture medium. Optimal increase in cell number of fractions 2-4 was achieved by the addition of augmentation agents phytohemagglutinin and recombinant interleukin-2 to culture medium, however, interleukin-2 alone failed to induce mitogenesis. Fractions 6-7, containing monocytes, were unstimulated.
dc.titleGrowth Characteristics Of Large Granular Lymphocytes In Vitro Upon Stimulation With Mitogens Phytohemagglutinin And Recombinant Interleukin-2
dc.typethesis
carrollscholars.object.degreeBachelor's
carrollscholars.object.departmentLife & Environmental Sciences
carrollscholars.object.disciplinesBiological Phenomena, Cell Phenomena, and Immunity; Cell Biology; Immunology and Infectious Disease
carrollscholars.legacy.itemurlhttps://scholars.carroll.edu/lifesci_theses/328
carrollscholars.legacy.contextkey12276006
carrollscholars.object.seasonSpring
dc.date.embargo12/31/1899 0:00


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