• Login
    View Item 
    •   Carroll Scholars Home
    • Life and Environmental Sciences
    • Life and Environmental Sciences Undergraduate Theses
    • View Item
    •   Carroll Scholars Home
    • Life and Environmental Sciences
    • Life and Environmental Sciences Undergraduate Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Growth Characteristics Of Large Granular Lymphocytes In Vitro Upon Stimulation With Mitogens Phytohemagglutinin And Recombinant Interleukin-2

    Thumbnail
    View/Open
    1988_MorinL_THS_000892.pdf (2.187Mb)
    Author
    Morin, Lisa
    Advisor
    John Christenson; Rev. Joseph Harrington; Rev. J. Eugene Peoples
    Date of Issue
    1988-04-01
    Metadata
    Show full item record
    URI
    https://scholars.carroll.edu/handle/20.500.12647/3070
    Title
    Growth Characteristics Of Large Granular Lymphocytes In Vitro Upon Stimulation With Mitogens Phytohemagglutinin And Recombinant Interleukin-2
    Type
    thesis
    Abstract
    A pilot experiment was performed in which peripheral blood lymphocytes were isolated from whole blood using the Ficoll-Hypaque technique. Percoll discontinuous-gradient centrifugation of peripheral blood mononuclear cells resulted in a 2-fold increase in natural killer cell activity coupled with a 3 to 4-fold increase in large granular lymphocytes. Morphology, function (NK activity), and phenotype of Percoll fractions 2-4, presumably large granular lymphocytes, were compared with that of lower density fractions 6-7 over a 28-day period. Augmentation agents phytohemagglutinin and recombinant interleukin-2 were added to the culture medium. Optimal increase in cell number of fractions 2-4 was achieved by the addition of augmentation agents phytohemagglutinin and recombinant interleukin-2 to culture medium, however, interleukin-2 alone failed to induce mitogenesis. Fractions 6-7, containing monocytes, were unstimulated.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
    Collections
    • Life and Environmental Sciences Undergraduate Theses

    Browse

    All of Carroll ScholarsCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    DSpace software copyright © 2002-2023  DuraSpace
    DSpace Express is a service operated by 
    Atmire NV