A Study Of Ibuprofen-Induced Renal Papillary Necrosis In Neonatal Mice Of The BALB/c db Strain

Abstract

It is now well established that renal papillary necrosis may be induced by a number of nonsteroidal anti-inflammatory agents. In this experiment, pregnant mice of the BALB/c db strain were intravenously injected with 0.1 ml of buffered Ibuprofen or buffer alone on days six, eleven, and sixteen of their gestational period. The control group was given 0.1 M sodium phosphate solution. The experimental group was given 69.73 mg/kg of Ibuprofen in 0.1 M sodium phosphate solution. Within twenty-four hours after birth, the pups were sacrificed and the left kidneys were removed. The kidneys were fixed, sectioned, and stained with hematoxylin and eosin. The slides were examined histologically. Comparative histological examination of representative sections of the kidneys revealed no significant difference between the tubules in the papillae of the two groups.