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    Construction of a chimeric antibody against group B streptococcus: Cloning the variable region of the mouse heavy chain gene

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    1989_JohnsonM_THS_000628.pdf (1.713Mb)
    Author
    Johnson, Mike
    Date of Issue
    1989-04-01
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/3063
    Title
    Construction of a chimeric antibody against group B streptococcus: Cloning the variable region of the mouse heavy chain gene
    Type
    thesis
    Abstract
    As a first step leading toward construction of a mouse-human chimeric antibody against Group B streptococcus, attempts were made to clone the heavy variable region gene from a murine hybridoma. The hybridoma was known to produce an anti-group B streptococcus monoclonal antibody. A conventional phagevector cloning approach was utilized as were the polymerase chain reaction and DNA size differentiation by electrophoresis. Attempts were unsuccessful, but a hybridoma gene library was created.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
    Collections
    • Life and Environmental Sciences Undergraduate Theses

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