• Login
    View Item 
    •   Carroll Scholars Home
    • Life and Environmental Sciences
    • Life and Environmental Sciences Undergraduate Theses
    • View Item
    •   Carroll Scholars Home
    • Life and Environmental Sciences
    • Life and Environmental Sciences Undergraduate Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Increased Production Of Pertussigen S1 Subunit Via Phage Expression And Insertion Of A Transcription Terminator

    Thumbnail
    View/Open
    1989_MottB_THS_000622.pdf (2.010Mb)
    Author
    Mott, Bradley
    Advisor
    John Addis; John Christenson; Barry Ferst
    Date of Issue
    1989-04-01
    Metadata
    Show full item record
    URI
    https://scholars.carroll.edu/handle/20.500.12647/3059
    Title
    Increased Production Of Pertussigen S1 Subunit Via Phage Expression And Insertion Of A Transcription Terminator
    Type
    thesis
    Abstract
    Increased production of the S1 subunit of the toxin secreted by Bordetella pertussis, the causitive agent of whooping cough, was attempted by two methods. The first method was to use phage to insert the sequence for the S1 subunit into IL coli so that this bacterium would produce the subunit. This method was used to replace an inefficient heat transduction method formerly used. The second method attempted to increase the S1 subunit production by inserting a termination sequence after the S1 subunit sequence. It was hoped that reading of the sequence would stop after the S1 sequence, disengage and go to the start of the sequence, thus eliminating the production of unwanted proteins. It was found that the phage expression system used did increase S1 subunit production. The insertion of the terminator to produce a new, more efficient construct, was not completed, so it remains unknown if this new construct would increase S1 subunit production.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
    Collections
    • Life and Environmental Sciences Undergraduate Theses

    Browse

    All of Carroll ScholarsCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    DSpace software copyright © 2002-2023  DuraSpace
    DSpace Express is a service operated by 
    Atmire NV