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    Molecular Cloning and Characterization of a 35 K Protein-Coding Gene that is Coregulated with the Gene(s) for the Outer Membrane Protein P.lla in Neisseria Gonorrhoeae

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    1989_WellsK_THS_000617.pdf (1.829Mb)
    Author
    Wells, Kathryn
    Advisor
    John Christenson; Jean Smith; Tomas Graman
    Date of Issue
    1989-04-01
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/3056
    Title
    Molecular Cloning and Characterization of a 35 K Protein-Coding Gene that is Coregulated with the Gene(s) for the Outer Membrane Protein P.lla in Neisseria Gonorrhoeae
    Type
    thesis
    Abstract
    A gene which coregulates the gene(s) that codes for the outer membrane protein P.ll in the bacterium Neisseria gonorrhoeae was cloned and characterized. As the cell expresses the P.ll protein, it no longer expresses the second protein; however, as the cell turns off the expression of P.ll, it then makes the second protein. A library of strain MS11 of the bacterium was constructed using the Lambda ZAP bacteriophage vector. This library was screened using a monospecific antiserum for the second protein. Molecular characterization of the positive clones followed.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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