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dc.contributor.advisorJohn Addis
dc.contributor.advisorRev. Joseph Harrington
dc.contributor.advisorNathalie Caulliez-Oppedahl
dc.contributor.authorHoiness, Marty
dc.date.accessioned2020-04-30T10:01:54Z
dc.date.available2020-04-30T10:01:54Z
dc.date.issued1991-04-01
dc.identifier.urihttps://scholars.carroll.edu/handle/20.500.12647/3039
dc.description.abstractIn this study, a gene construct containing 4.6 Kb of Ren-2 5' flanking sequence fused to either the gene encoding /3-galactosidase or to the T antigen was tested for its ability to confer a tissue specific expression profile in transgenic mice. Eight transgenic lines were analyzed for the presence of the transgene by Southern blotting techniques and by dot blot hybridization. Northern blot hybridization of total RNA isolated from tissues was performed to assay for the presence of transgene transcripts. No detectable transgene expression was observed in 5 transgenic lines. Testicular transgene expression was evident in 2 transgenic lines, and tissue specific expression was observed in 1 line. Application for the development of an assay for studying renin development during fetal development is discussed.
dc.titleStudies Of The Murine Ren-2 Promoter Region In Transgenic Mice
dc.typethesis
carrollscholars.object.degreeBachelor's
carrollscholars.object.departmentLife & Environmental Sciences
carrollscholars.object.disciplinesGenetics
carrollscholars.legacy.itemurlhttps://scholars.carroll.edu/lifesci_theses/297
carrollscholars.legacy.contextkey12098794
carrollscholars.object.seasonSpring
dc.date.embargo12/31/1899 0:00


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