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    Immunohistochemical Localization of Tubulin in the Somite of the Early Chick Embryo

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    1991_ScharfeS_THS_000471.pdf (1.322Mb)
    Author
    Scharfe, Stace
    Advisor
    John Addis; John Christenson; Joan Stottlemyer
    Date of Issue
    1991-04-01
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/3036
    Title
    Immunohistochemical Localization of Tubulin in the Somite of the Early Chick Embryo
    Type
    thesis
    Abstract
    In this experiment I attempted to devise a procedure for the localization of the cytoskeletal protein tubulin in the somite of the early chick embryo. I also checked the distribution of tubulin in the early chick somite for any possible relationship to the epithelial-mesenchymal transition that occurs in these somitic ceils. Newly fertilized chicken eggs were incubated for approximately 48 hr; then the embryos were removed from the egg, fixed, embedded, and frozen for cryostat sectioning. After sectioning the embryos, I attempted to localize tubulin by attaching a primary antibody to the tubulin molecule followed by a secondary antibody and an enzyme (peroxidase). A peroxidase substrate was then introduced to the tissue, and the enzyme reacted with it to produce a colored reaction product which served as the indicator for the presence and distribution of the tubulin.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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