Immunohistochemical Localization of Tubulin in the Somite of the Early Chick Embryo

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Authors
Scharfe, Stace
Advisor
John Addis
John Christenson
Joan Stottlemyer
Editor
Date of Issue
1991-04-01
Subject Keywords
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Citation
Series/Report No.
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Title
Immunohistochemical Localization of Tubulin in the Somite of the Early Chick Embryo
Other Titles
Type
thesis
Description
Abstract
In this experiment I attempted to devise a procedure for the localization of the cytoskeletal protein tubulin in the somite of the early chick embryo. I also checked the distribution of tubulin in the early chick somite for any possible relationship to the epithelial-mesenchymal transition that occurs in these somitic ceils. Newly fertilized chicken eggs were incubated for approximately 48 hr; then the embryos were removed from the egg, fixed, embedded, and frozen for cryostat sectioning. After sectioning the embryos, I attempted to localize tubulin by attaching a primary antibody to the tubulin molecule followed by a secondary antibody and an enzyme (peroxidase). A peroxidase substrate was then introduced to the tissue, and the enzyme reacted with it to produce a colored reaction product which served as the indicator for the presence and distribution of the tubulin.
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Degree Awarded
Bachelor's
Semester
Spring
Department
Life & Environmental Sciences