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    Enzyme Cytochemical Localization Of Na,K-ATPase And Immunohistochemical Localization Of Vinculin In Frog And Mouse Liver Tissue

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    Author
    McInnis, Douglas
    Advisor
    John Addis; Rev. Joseph Harrington; Robert Swartout
    Date of Issue
    1992-04-01
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/3029
    Title
    Enzyme Cytochemical Localization Of Na,K-ATPase And Immunohistochemical Localization Of Vinculin In Frog And Mouse Liver Tissue
    Type
    thesis
    Abstract
    Means of localizing molecules are essential in contemporary biological research attempting to unlock the mysteries of how cells function, interact, and go wrong. Enzyme cytochemistry and immunohistochemistry are two methods which utilize different characteristics of given molecules to localize them in tissue. The objective of this study was to localize two proteins, Na,K-ATPase and vinculin, in frog and mouse liver sections using these methods. Na,KATPase activity contributes to overall p-nitrophenylphosphatase (jpNPPase) activity, and sites of p-NPPase activity were determined using an enzyme cytochemical method. Sites of p-NPPase activity were thus extrapolated to indicate regions of probable Na,K-ATPase activity. Vinculin was localized immunohistochemically using an antibody prepared specifically against the vinculin molecule. Liver sections mounted on glass slides were subjected to appropriate reaction conditions to carry out either the enzyme cytochemical method for p-NPPase, or the immunohistochemical method for vinculin. Results of the vinculin localization were inconclusive, but results of the p-NPPase localization enabled probable identification of Na,K-ATPase at lateral hepatocyte membranes, along the lining of the sinusoids, and associated with the bile canalicular membranes.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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