• Login
    View Item 
    •   Carroll Scholars Home
    • Life and Environmental Sciences
    • Life and Environmental Sciences Undergraduate Theses
    • View Item
    •   Carroll Scholars Home
    • Life and Environmental Sciences
    • Life and Environmental Sciences Undergraduate Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Characterization And Cloning Of Coxiella burnetii

    Thumbnail
    View/Open
    1992_McLeanJ_THS_000453.pdf (2.324Mb)
    Author
    McLean, Janelle
    Advisor
    Rev. Joseph Harrington; Arthur Westwell; John Hart
    Date of Issue
    1992-04-01
    Metadata
    Show full item record
    URI
    https://scholars.carroll.edu/handle/20.500.12647/3028
    Title
    Characterization And Cloning Of Coxiella burnetii
    Type
    thesis
    Abstract
    Coxiella burnetii, an obligate intracellular parasite, is the etiological agent of the disease QFever, which can occur in acute and chronic forms. The acute form is rarely serious, but chronic Q-Fever may result in endocarditis, which is usually fatal. It is therefore of interest to determine which strains of Coxiella burnetii lead to the acute versus the endocarditis-associated sickness, and what the mechanisms involved in each are. This past summer at Rocky Mountain Laboratories in Hamilton, Montana, under the direction of Dr.Ted Hackstadt,I began to research the Coxiella burnetii 9-Mile strain, which is associated with the acute form of the disease. DNA characterization of this bacterium by restriction mapping was performed as well as attempts with antibodies to determine which proteins were cloned into recombinants. These could be compared to those of endocarditis-associated strains. With lambda gtll as a cloning and expression vector, DNA was inserted into various plasmids, proteins of the systems were expressed, and ligations and transformations of plasmids with Escherichia coli strains were carried out. These transformants were then used in plasmid mini-preparations for isolation and identification of the DNA we were successful in cloning.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
    Collections
    • Life and Environmental Sciences Undergraduate Theses

    Browse

    All of Carroll ScholarsCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    DSpace software copyright © 2002-2023  DuraSpace
    DSpace Express is a service operated by 
    Atmire NV