• Login
    View Item 
    •   Carroll Scholars Home
    • Life and Environmental Sciences
    • Life and Environmental Sciences Undergraduate Theses
    • View Item
    •   Carroll Scholars Home
    • Life and Environmental Sciences
    • Life and Environmental Sciences Undergraduate Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    The Effect Of Retinoic Acid On Normal Human Foreskin Epithelial Cells And HPV-Immortalized Cells Grown In Organotypic Raft Cultures

    Thumbnail
    View/Open
    1992_WhittenburgK_THS_000446.pdf (2.058Mb)
    Author
    Whittenburg, Kristin
    Advisor
    Rev. Joseph Harrington; John Addis; Brent Northup
    Date of Issue
    1992-04-01
    Metadata
    Show full item record
    URI
    https://scholars.carroll.edu/handle/20.500.12647/3025
    Title
    The Effect Of Retinoic Acid On Normal Human Foreskin Epithelial Cells And HPV-Immortalized Cells Grown In Organotypic Raft Cultures
    Type
    thesis
    Abstract
    Previous studies have shown that epithelial cells immortalized by human papillomaviruses (HPVs) at late passages were more resistant to the effects of retinoic acid than late passage normal keratinocytes. Some of these late passage HPV-immortalized cells, however, lose the ability to form a cornified envelope with continued culturing. For this reason, early passage normal keratinocytes and early passage HPV-immortalized cells were studied to see if they also showed this resistance to retinoic acid. Normal epithelial cells and cells immortalized by HPVs were grown in organotypic raft cultures and treated with varying amounts of retinoic acid, which normally blocks terminal differentiation in keratinocytes. The cultures were grown for two weeks, fixed, sectioned, and stained with either hematoxylin and eosin (H&E) or an antibody against the keratin K1. The HPV-immortalized cell lines were found to be more resistant to the action of retinoic acid than the normal cells. The same cell lines were also grown in culture plates in the same concentrations of retinoic acid. These cultures were counted daily for one week, and the results were used to construct growth curves and viability charts. It was found that the normal and HPV-immortalized cells show the same response to the growth inhibitory effects of retinoic acid. In addition, the viability of all cell lines was not significantly reduced by the higher concentrations of retinoic acid This showed that the differences in morphology seen in the raft cultures were probably due to a different mechanism than that which causes growth inhibition.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
    Collections
    • Life and Environmental Sciences Undergraduate Theses

    Browse

    All of Carroll ScholarsCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    DSpace software copyright © 2002-2021  DuraSpace
    DSpace Express is a service operated by 
    Atmire NV