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    The Detection Of Glycoproteins In The Plasma Membrane Of Dictyostelium discoideum

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    1992_WoodC_THS_000445.pdf (1.161Mb)
    Author
    Wood, Chris
    Advisor
    John Christenson; Rev. Jeremiah Lowney; Rev. Joseph Harrington
    Date of Issue
    1992-04-01
    Subject Keywords
    Dictyostelium discoideum
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/3024
    Title
    The Detection Of Glycoproteins In The Plasma Membrane Of Dictyostelium discoideum
    Type
    thesis
    Abstract
    A clone of Dictyostelium discoideum strain AX-3 was cultured up to its amoeboid stage. The glycoproteins in the plasma membrane of strain AX-3 were then isolated and purified. The proteins were fractionated by SDS polyacrylamide gel electrophoresis and then transferred to a nitrocellulose sheet by western blotting. The resulting nitrocellulose blot was used to detect glycoproteins with biotinylated concanavalin A and avidin-alkaline phosphatase. The results suggest there are mannose containing glycoproteins in the plasma membrane of strain AX-3.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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