The Detection Of Glycoproteins In The Plasma Membrane Of Dictyostelium discoideum

Abstract

A clone of Dictyostelium discoideum strain AX-3 was cultured up to its amoeboid stage. The glycoproteins in the plasma membrane of strain AX-3 were then isolated and purified. The proteins were fractionated by SDS polyacrylamide gel electrophoresis and then transferred to a nitrocellulose sheet by western blotting. The resulting nitrocellulose blot was used to detect glycoproteins with biotinylated concanavalin A and avidin-alkaline phosphatase. The results suggest there are mannose containing glycoproteins in the plasma membrane of strain AX-3.