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    The Effect of PKC 5 Inhibitors on PMA-induced Apoptosis in Xenopus laevis Splenocytes

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    2003_CraterA_THS_000825.pdf (2.111Mb)
    Author
    Crater, Annie
    Advisor
    Gerald Shields; Grant Hokit; Ron Wilde
    Date of Issue
    2003-04-01
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/2978
    Title
    The Effect of PKC 5 Inhibitors on PMA-induced Apoptosis in Xenopus laevis Splenocytes
    Type
    thesis
    Abstract
    Protein kinase C (PKC) controls apoptosis in amphibian systems such as that of the South African clawed frog, Xenopus laevis. The effects of cancer inducing agents like phorbol 12-myristate 13-acetate (PMA) when combined with Ca2+ independent PKC inhibitors on programmed cell death were studied in A laevis splenocytes. Regulation of PMA-activated cells was observed via Annexin V-FITC and propidium iodide assays, flow cytometry, and Western blot analysis. Results indicate the PKC inhibitors reduced both apoptosis and cell growth in PMA-stimulated X. laevis splenocytes compared to PMA alone. These results are valuable to future mammalian cancer research. By identifying the various apoptotic mechanisms utilized by amphibian cells, researchers may become more informed regarding amphibians’ increased resistance to cancer and apply such knowledge to mammalian systems.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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