Separation of Underivatized Amino Acids and Dipeptides Using 1- Butylimidazolium Based Stationary Phase and UV Detection

Abstract

Isocratic elution of aromatic amino acids was achieved using a butyl-imidazolium based stationary phase and UV detection. For this study butyl-imidazolium bromide was bonded to a silica substrate and packed into an HPLC column. This study focused on the effects of ionic strength and organic modifier on retention. Retention of aromatic dipeptides was also explored to gain further insight into the retention mechanisms exhibited by this new stationary phase. The stationary phase was found to exhibit both reverse-phase and ion-exchange retention mechanisms.