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    Separation of Underivatized Amino Acids and Dipeptides Using 1- Butylimidazolium Based Stationary Phase and UV Detection

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    2004_BlackburnA_THS_000778.pdf (2.398Mb)
    Author
    Blackburn, Amanda
    Advisor
    Kyle Strode; Ron Wilde; Debra Bernardi
    Date of Issue
    2004-04-01
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/2967
    Title
    Separation of Underivatized Amino Acids and Dipeptides Using 1- Butylimidazolium Based Stationary Phase and UV Detection
    Type
    thesis
    Abstract
    Isocratic elution of aromatic amino acids was achieved using a butyl-imidazolium based stationary phase and UV detection. For this study butyl-imidazolium bromide was bonded to a silica substrate and packed into an HPLC column. This study focused on the effects of ionic strength and organic modifier on retention. Retention of aromatic dipeptides was also explored to gain further insight into the retention mechanisms exhibited by this new stationary phase. The stationary phase was found to exhibit both reverse-phase and ion-exchange retention mechanisms.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
    Collections
    • Life and Environmental Sciences Undergraduate Theses

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