Developing Proteomics Techniques for Glycosylated Proteins

Abstract

The ability to study proteins and their role in cell function is a central area of scientific study. To learn more about proteins and glycosylation, a form of posttranslational modification, a means of detecting specifically for glycosylation is necessary. The modification of an existing dye to meet this need was the goal ofthe project. N-benzyl-4-nitroaniline and methyl ((4-(4-nitrophenyl)amino)methyl)benzoate, two intermediate compounds in the synthesis, were created and purified.