• Login
    View Item 
    •   Carroll Scholars Home
    • Life and Environmental Sciences
    • Life and Environmental Sciences Undergraduate Theses
    • View Item
    •   Carroll Scholars Home
    • Life and Environmental Sciences
    • Life and Environmental Sciences Undergraduate Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Molecular Diagnosis of Fragile X Syndrome and AGG Interspersion Analysis

    Thumbnail
    View/Open
    1997_HemmerS_THS_000327.pdf (3.164Mb)
    Author
    Hemmer, Scott
    Advisor
    Marilyn Schendel; Linda Beischel; Grant Hokit
    Date of Issue
    1997-04-01
    Metadata
    Show full item record
    URI
    https://scholars.carroll.edu/handle/20.500.12647/2892
    Title
    Molecular Diagnosis of Fragile X Syndrome and AGG Interspersion Analysis
    Type
    thesis
    Abstract
    Two molecular techniques used for studying Fragile X syndrome were tested in this study. The first technique detemines the number of CGG repeats in the FRAXA repeat region. The second examines the number of CGG repeats without AGG interspersions. The first technique used PCR (polymerase chain reaction) to specifically amplify DNA from the repeat region that had been cut by the restriction enzyme Hind III. Then the amplified DNA size was determined using agarose gels stained with ethidium bromide The first technique demonstrated that it could accurately determine the number of CGG repeats in most individuals. Thus, it shows promise in identifying individuals that do not need further fragile X testing. The second technique also amplified Hind III cut DNA. This DNA was then purified and cut with the restriction enzyme Mnll. These fragments were then also separated on an agarose gel and stained with ethidium bromide. The second technique demonstrated an ability to identify large regions of pure CGG repeats at the FRAXA site. This technique may in the future provide a method of determining premutation stability.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
    Collections
    • Life and Environmental Sciences Undergraduate Theses

    Browse

    All of Carroll ScholarsCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    DSpace software copyright © 2002-2023  DuraSpace
    DSpace Express is a service operated by 
    Atmire NV