• Login
    View Item 
    •   Carroll Scholars Home
    • Life and Environmental Sciences
    • Life and Environmental Sciences Undergraduate Theses
    • View Item
    •   Carroll Scholars Home
    • Life and Environmental Sciences
    • Life and Environmental Sciences Undergraduate Theses
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Acid Phosphatase Content And Acidity Of The Perialgal Vacuole Of Ephydatia muelleri

    Thumbnail
    View/Open
    1997_MinerC_THS_000322.pdf (2.164Mb)
    Author
    Miller, Camette
    Advisor
    John Addis; Marilyn Schendel; Brent Northup
    Date of Issue
    1997-04-01
    Subject Keywords
    freshwater sponge, Ephydatia muelleri, Chlorella
    Metadata
    Show full item record
    URI
    https://scholars.carroll.edu/handle/20.500.12647/2890
    Title
    Acid Phosphatase Content And Acidity Of The Perialgal Vacuole Of Ephydatia muelleri
    Type
    thesis
    Abstract
    Freshwater sponges containing symbiotic algae can serve as a model for the study of relationships in which a host and an intracellular symbiont benefit. In freshwater sponges, symbiotic algae belonging to the genus, Chlorella, are housed in large cytoplasmic vacuoles, called perialgal vacuoles. I used microscopic techniques in an effort to determine whether the perialgal vacuoles ofthe freshwater sponge, Ephydatia muelleri, (1) contain active acid phosphatase, a lysosomal enzyme and (2) are acidic. Whereas the postcoupling method for localizing active acid phosphatase gave equivocal results, the Gomori method for localizing the enzyme revealed that few of the perialgal vacuoles possesed active enzyme. The fluorescence of LysoSensor pH-sensitive probes suggested that the perialgal vacuoles are not strongly acidic and may have a pH of between -5.5 and —8.0. The latter conclusion is considered tentative, however, since the precise location of the fluorescing LysoSensor probe within the perialgal vacuoles could not be ascertained.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
    Collections
    • Life and Environmental Sciences Undergraduate Theses

    Browse

    All of Carroll ScholarsCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    DSpace software copyright © 2002-2023  DuraSpace
    DSpace Express is a service operated by 
    Atmire NV