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    Ultrasound Transfection A Gene Transfer Assay for Increased Cell Permeability without Impacting Cell Viability

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    2000_AndersonB_THS_000237.pdf (2.283Mb)
    Author
    Anderson, Brianne
    Advisor
    Marilyn Schendel; Rev. Joseph Harrington; Steven Visuri
    Date of Issue
    2000-04-01
    Metadata
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/2835
    Title
    Ultrasound Transfection A Gene Transfer Assay for Increased Cell Permeability without Impacting Cell Viability
    Type
    thesis
    Abstract
    In this study, NIH/3T3 cells were permeated with both chemical and physical methods for transient gene transfection. Cationic lipid transfection was used as the mode of chemical transfection with Transfectam used as the transfection reagent and pSV-P- galactosidase as the control vector. After addition of transfection agents, cells were exposed to ultrasound with either of two ultrasound probe-type sonicators: either a 20 kHz, 130 W Vibra-Cell Ultrasonic Processor or a 20 kHz, 400 W Vibra-Cell Ultrasonic Processor. With the 130 W Vibra-Cell Ultrasonic Processor, no correlation could be made between transfection rates and ultrasound exposure (duration or power). Using the 400 W Vibra-Cell Ultrasonic Processor, it was determined that a 1% amplitude for 30 seconds may represent the optimal set of conditions to enhance cell permeability and that the viability of the cells decreases at higher levels of amplitude.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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