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    GENERATION OF pFastBacl-HT AND ISOLATION OF ErbB-2, ErbB-3 AND ErbB-4 cDNAs by RT-PCR

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    2000_DiekhansS_THS_000231.pdf (1.938Mb)
    Author
    Diekhans, Stacy
    Advisor
    John Christenson; John Addis; Mary Keeffe
    Date of Issue
    2000-04-01
    Subject Keywords
    ErbB receptor tyrosine kinase subfamily
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    URI
    https://scholars.carroll.edu/handle/20.500.12647/2831
    Title
    GENERATION OF pFastBacl-HT AND ISOLATION OF ErbB-2, ErbB-3 AND ErbB-4 cDNAs by RT-PCR
    Type
    thesis
    Abstract
    Over-expression of the ErbB receptor tyrosine kinase subfamily has been implicated in various types of human cancers. In the current study, ErbB-2, ErbB-3, and ErbB-4 tyrosine kinase domain cDNAs were isolated by RT-PCR and subcloned into a pFastBac-HT vector created through genetic engineering for the purpose of placing a his- tag on the ErbB protein receptors for purification. In isolating the ErbB-4 cDNA, an alternate receptor containing a 48 base-pair deletion in the tyrosine kinase region of the receptor was recovered. This result confirms an earlier report of an alternate splicing pattern in the ErbB-4 mRNA. Isolation of the ErbB TKD cDNAs allows for further research for the development of a diagnostic test of cancer tissues for over-expression of the ErbB receptors similar to the Herceptest, which tests for over-expression of ErbB-2.
    Degree Awarded
    Bachelor's
    Semester
    Spring
    Department
    Life & Environmental Sciences
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    • Life and Environmental Sciences Undergraduate Theses

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